To ensure accuracy and precision when pipetting small volumes, proper technique and equipment use are essential. The following are recommended procedures for pipetting tiny volumes: Use the correct pipette: Select a pipette made especially for pipetting tiny amounts of liquid. Compared to pipettes made for higher volumes, these pipettes have finer graduations and smaller plunger sizes. Use low-retention pipette tips: When pipetting small volumes, it is important to minimize liquid retention, which is why low-retention pipette tips are specially designed to do just that. Usually composed of a different kind of polypropylene, these tips are lubricated with silicone to lower surface tension and keep liquid from sticking to the inside of the tip. Pre-wet the pipette tip: Reduce liquid retention and increase accuracy by pre-wetting the pipette tip with a small amount of the liquid you plan to pipette. This is particularly crucial for liquids that are sticky or viscous. Use a slow pipetting speed: When dispensing small volumes, use the pipette slowly. This will lessen the possibility of air bubbles forming by allowing the liquid more time to flow into and out of the tip. Pause before dispensing: As you aspirate the liquid, give it a brief moment of rest before dispensing. This will guarantee that you are dispensing the right amount by allowing the liquid to settle in the tip. Dispense at an angle: One should dispense the liquid at a 45-degree angle to the vessel's surface. By doing this, you can lessen the chance that the liquid will drip or fall off the side of the container. Touch the pipette tip to the side of the vessel: To eliminate any leftover liquid, touch the pipette tip to the vessel's side after dispensing the liquid. By doing this, you can be sure that the right amount is being dispensed. Change pipette tips frequently: Often replace pipette tips, particularly when pipetting small volumes. This is due to the possibility that minute liquid particles may stick to the inside of the tip, impairing the precision of later pipetting. Calibrate your pipette: Make sure your pipette is dispensing the right amount of liquid by calibrating it on a regular basis. This is particularly crucial for pipetting tiny volumes. Practice proper technique: Use the right pipetting technique to guarantee accurate and consistent outcomes. This entails using a proper pipette grip, applying constant pressure to the plunger, and avoiding jerky motions. You can increase the precision and accuracy of pipetting small volumes by adhering to these best practices, which will guarantee consistent and repeatable outcomes in your laboratory tests. For more information, please click:https://www.cqscopelab.com/pipette-tips...

Selecting the right pipette tip for your application is essential to ensuring accurate and precise results. There are several considerations to make when selecting a pipette. These include the type of pipette you're using, the volume of liquid you're moving, the viscosity of the liquid, and the application's purity specifications. Type of pipette The two essential types of pipettes are air displacement and positive displacement. An air displacement pipette, which aspirates and dispenses liquid by creating a vacuum, is the most widely used type of pipette. Liquids containing volatile organic compounds (VOCs) or viscous liquids are commonly pipetted using positive displacement pipettes, which function by forcing liquid through the pipette with the help of a piston. Different types of pipettes require different types of pipette tips. For example, universal pipette tips are required for air displacement pipettes, while positive displacement pipettes require specially designed pipette tips. Volume of liquid Pipette tips come in multiple volumes, varying from 0.1 µL to 5 mL. It's important to choose a pipette tip that matches the volume of the liquid you're transferring. A pipette tip that is too large can yield inconsistent results, and one that is too small can make it difficult to aspirate and dispense the necessary volume of liquid. Viscosity of the liquid The consistency of the liquid may also have an impact on selecting a suitable pipette tip. Viscous liquids, such as glycerol or honey, are difficult to aspirate and dispense precisely. For viscous liquids, a low-retention pipette tip is recommended. Low-retention pipette tips have a special coating that helps reduce the amount of liquid that adheres to the inside of the tip. Purity requirements Pipette tips that are sterile and free of pyrogens are necessary for certain applications, like molecular biology and cell culture. Bacteria and other microorganisms are absent from sterile pipette tips, but endotoxins—substances produced by bacteria that induce fever—are present in pyrogen-free pipette tips. By considering the above-mentioned factors, you can choose the best pipette tip for your application and ensure accurate and precise results.For more information，please click：https://www.cqscopelab.com/pipette-tips...