Selecting the right pipette tip for your application is essential to ensuring accurate and precise results. There are several considerations to make when selecting a pipette. These include the type of pipette you're using, the volume of liquid you're moving, the viscosity of the liquid, and the application's purity specifications.
Type of pipette
The two essential types of pipettes are air displacement and positive displacement. An air displacement pipette, which aspirates and dispenses liquid by creating a vacuum, is the most widely used type of pipette. Liquids containing volatile organic compounds (VOCs) or viscous liquids are commonly pipetted using positive displacement pipettes, which function by forcing liquid through the pipette with the help of a piston. Different types of pipettes require different types of pipette tips. For example, universal pipette tips are required for air displacement pipettes, while positive displacement pipettes require specially designed pipette tips.
Volume of liquid
Pipette tips come in multiple volumes, varying from 0.1 µL to 5 mL. It's important to choose a pipette tip that matches the volume of the liquid you're transferring. A pipette tip that is too large can yield inconsistent results, and one that is too small can make it difficult to aspirate and dispense the necessary volume of liquid.
Viscosity of the liquid
The consistency of the liquid may also have an impact on selecting a suitable pipette tip. Viscous liquids, such as glycerol or honey, are difficult to aspirate and dispense precisely. For viscous liquids, a low-retention pipette tip is recommended. Low-retention pipette tips have a special coating that helps reduce the amount of liquid that adheres to the inside of the tip.
Purity requirements
Pipette tips that are sterile and free of pyrogens are necessary for certain applications, like molecular biology and cell culture. Bacteria and other microorganisms are absent from sterile pipette tips, but endotoxins—substances produced by bacteria that induce fever—are present in pyrogen-free pipette tips.
By considering the above-mentioned factors, you can choose the best pipette tip for your application and ensure accurate and precise results.For more information，please click：https://www.cqscopelab.com/pipette-tips
